Effects of replacing Na selenite in laying hen feed with selenized glucose on production performance, egg quality, egg selenium content, microbial population, immunological response, antioxidant enzymes, and fatty acid composition.

This study aimed to explore the effects of selenized glucose (SeGlu) and Na selenite supplementation on various aspects of laying hens such as production performance, egg quality, egg Se concentration, microbial population, antioxidant enzymes activity, immunological response, and yolk fatty acid profile. Using a 2 × 2 factorial design, 168 laying hens at 27-wk of age were randomly divided into 4 treatment groups with 7 replications. Se source (Na selenite and SeGlu) and Se level (0.3 and 0.6 mg/kg) were used as treatments. When 0.3 mg SeGlu/kg was compared to 0.3 mg Na selenite/kg, the interaction findings revealed that 0.3 mg SeGlu/kg increased egg production percent and shell ash (P < 0.05). When compared to 0.3 mg Na selenite/kg, dietary supplementation with 0.3 and 0.6 mg SeGlu/kg resulted in an increase in albumen height, Haugh unit, and yolk color of fresh eggs (P < 0.05). SeGlu enhanced albumen height, Haugh unit, shell thickness (P < 0.01), albumen index, yolk share, specific gravity, shell ash (P < 0.05) of fresh eggs and shell thickness (P < 0.05) of stored eggs as compared to Na selenite. The interaction showed that 0.6 mg SeGlu/kg enhanced yolk Se concentration while decreasing malondialdehyde levels in fresh egg yolk (P < 0.05). SeGlu enhanced Se concentration in albumen and glutathione peroxidase activity in plasma (P < 0.05) as compared to Na selenite. 0.6 mg Se/kg increased lactic acid bacteria, antibody response to sheep red blood cells, and lowered ∑n-6 PUFA/ ∑n-3 PUFA ratio (P < 0.05). As a result, adding SeGlu to the feed of laying hens enhanced egg production, egg quality, egg Se concentration, fresh yolk lipid oxidation, and glutathione peroxidase enzyme activity.

The effect of replacing sodium selenite with selenium-chitosan in laying hens on production performance, egg quality, egg selenium concentration, microbial population, immunological response, antioxidant enzymes, and fatty acid composition.

The purpose of this study was to investigate into the effects of Se-chitosan and Na selenite supplementation on laying hen production performance, egg quality, egg Se concentration, microbial population, immunological response, antioxidant enzymes activity, and yolk fatty acid profile. Using a 2 × 2 factorial design, 168 27-wk-old laying hens were randomly divided into 4 treatment groups and 7 replications. Se source (Na selenite and Se-chitosan) and Se level (0.3 and 0.6 mg/kg) were used as treatments. Se-chitosan enhanced egg production percentage and egg mass (P < 0.05) when compared with Na selenite. There was an interaction, with 0.6 mg Se-chitosan/kg causing an increase in albumen height, Haugh unit, albumen index, and shell thickness of fresh eggs (P < 0.05). Se-chitosan increased yolk share, yolk color, and shape index of fresh eggs and shape index, albumen index, albumen height, Haugh unit, yolk color, shell thickness, and specific gravity of stored eggs (P < 0.05). The interaction showed that, 0.6 mg Se-chitosan/kg increased albumen Se concentration and decreased the level of malondialdehyde (MDA) in fresh egg yolk compared with 0.3 and 0.6 mg Na selenite/kg (P < 0.05). When compared with Na selenite, Se-chitosan increased the Se concentration in the yolk and decreased level of MDA in stored egg yolk (P < 0.01). When compared with Na selenite, Se-chitosan reduced coliforms (P < 0.01), increased lactic acid bacteria, and the lactic acid bacteria/coliform ratio (P < 0.05). Se-chitosan supplementation increased antibody response to sheep red blood cells and IgM titers and the activities of glutathione peroxidase and superoxide dismutase in plasma (P < 0.05). Furthermore, compared with Na selenite, supplementing diets with Se-chitosan decreased ∑ n-6 PUFA/∑ n-3 PUFA ratio (P < 0.01). In conclusion, Se-chitosan supplementation of laying hen feed improved production performance, egg quality, egg Se concentration, yolk lipid oxidation, microbial population, immune response, antioxidant enzymes activity, and yolk fatty acid profile, with 0.6 mg Se-chitosan/kg supplementation being optimal.